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Protein Biochemistry
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Rhodamine Phalloidin (14uM in methanol)
Rhodamine Phalloidin (14uM in methanol) from Cytoskeleton, Inc.
(1)
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Citations:
(127)
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Cytoskeleton, Inc. for
Rhodamine Phalloidin (14uM in methanol)
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Product Specs
Item
Rhodamine Phalloidin (14uM in methanol)
Company
Cytoskeleton, Inc.
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
PHDR1
Quantity
1 x 500 µl
Brand/Trademark
Cytoskeleton, Inc
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Cytoskeleton, Inc.
1830 S. Acoma St.
Denver
,
Colorado
80223
United States
Phone
: 303 322-2254
303 322-2257
Company Profile
Website:
http://cytoskeleton.com/
Citations (127)
(61)
A Whole Genome-Wide Arrayed CRISPR Screen in Primary Organ Fibroblasts to Identify Regulators of Kidney Fibrosis
SLAS discovery : advancing life sciences R & D
July 1, 2020
Turner RJ, Golz S, Wollnik C, Burkhardt N, Sternberger I, Andag U, Cornils H.
transforming growth factor-β1 (TGF-1)
human
(
A7481
) were purchased from
Sigma
. Design rhodamine phalloidin (
PHDR1
) was obtained from
Cytoskeleton
(tebu-bio, Offenbach, Germany).
DRAQ5
(
DR51000
) was obtained from
Biostatus
(62)
Biofunctionalization of Microgroove Surfaces with Antibacterial Nanocoatings
BioMed Research International
June 17, 2020
Yingzhen Lai, Zhiqiang Xu, Jiang Chen, Renbin Zhou, Jumei Tian, Yihuang Cai
for 30 min using a secondary antibody (
1 : 32
) and rhodamine-labeled phalloidin (
Cytoskeleton
Cat. #
PHDR1
) at
1 : 400
for 30 min. For nuclear fluorescence, 6-diamidino2-phenylindole (DAPI) (Sigma-Aldrich) was
(63)
TIMP1 preserves the blood-brain barrier through interacting with CD63/integrin
β
1 complex and regulating downstream FAK/RhoA signaling
Acta pharmaceutica Sinica. B
June 1, 2020
Tang J, Kang Y, Huang L, Wu L, Peng Y.
with PBS at 5-min intervals. F-actin
staining
was performed by using Rhodamine-Phalloidin (100 nmol/L,
PHDR1
,
Cytoskeleton
, Denver, CO, USA) at room temperature for 1 h. HBMECs were then incubated with Alexa488-anti-rabbit
(64)
Copine A regulates the size and exocytosis of contractile vacuoles and postlysosomes in
Dictyostelium
FEBS Open Bio
June 1, 2020
Elise M. Wight, Amber D. Ide, Cynthia K. Damer
antibody as described above, and then additionally stained with 100 nm rhodamine phalloidin (
Cytoskeleton
,
PHDR1
). The number of PLs and the number of PLs coated in a complete coat of actin were counted for each cell.
(65)
Bioactive poly (methyl methacrylate) bone cement for the treatment of osteoporotic vertebral compression fractures
Theranostics
May 17, 2020
Jinjin Zhu, Shuhui Yang, Kaiwen Cai, Shuo Wang, Zhiye Qiu, Junfei Huang, Guoqiang Jiang, Xiumei Wang, Xiangqian Fang
After the removal of this solution, the cells were stained with rhodamine-phalloidin (
1:300
) (cat. no.
PHDR1
;
Cytoskeleton
, Denver, CO, USA) at 37°C for 40 min and washed with PBS. After
staining
with 4', 6-diamidino-2-phenylindole
(66)
Lysine Acetylation Reshapes the Downstream Signaling Landscape of Vav1 in Lymphocytes
Cells
March 4, 2020
Sonia Rodríguez-Fdez, Lucía Fernández-Nevado, L. Francisco Lorenzo-Martín, Xosé R. Bustelo
(Cat. number CP06; Merck-Calbiochem, Burlington, MA, USA). Rhodamine-labeled phalloidin (Cat. number
PHDR1
) was obtained from
Cytoskeleton
(Denver, CO, USA). 2.3.
Cell Culture
Jurkat cells
were obtained from
(67)
The effect of fluid shear stress on fibroblasts and stem cells on plane and groove topographies
Cell Adhesion & Migration
January 17, 2020
Xing Lei, Bin Liu, Hao Wu, Xiao Wu, Xiu-Li Wang, Yue Song, Shuai-Shuai Zhang, Jun-Qin Li, Long Bi, Guo-Xian Pei
F0257
;
1:100
) at 37°C for 1 h. Subsequently, F-actin was stained with phalloidin-TRITC (
Cytoskeleton
;
PHDR1
; 100nM) and the nucleus with DAPI (
Thermo
;
P36931
; undiluted). All specimens were then examined under
(68)
EZH2 Supports Osteoclast Differentiation and Bone Resorption Via Epigenetic and Cytoplasmic Targets
Journal of Bone and Mineral Research
January 1, 2020
Juraj Adamik, Sree H. Pulugulla, Peng Zhang, Quanhong Sun, Konstantinos Lontos, David A. Macar, Philip E. Auron, Deborah L. Galson
(
D21490
,
Life technologies
) was used to visualize nuclei. F-actin was stained with rhodamine phalloidin (
PHDR1
,
Cytoskeleton Inc
.). Florescent images were captured using Olympus Fluoview 1000 and
Nikon
AIR Inverted
(69)
MLK3 Is Associated With Poor Prognosis in Patients With Glioblastomas and Actin Cytoskeleton Remodeling in Glioblastoma Cells
Frontiers in oncology
January 1, 2020
Zhu Y, Sun JM, Sun ZC, Chen FJ, Wu YP, Hou XY.
(#
A-11029
), and goat anti-rabbit IgG-Alexa Fluor 594 (#
A11037
) were obtained from
Invitrogen
.
Phalloidin
(#
PHDR1
) was obtained from
Cytoskeleton
. Full-length
human
MLK3
cDNA (puc19-hMAP3K11, #
HG11067-U
) was obtained
(70)
Osteogenic differentiation of murine mesenchymal stem cells by combination of surface topography and uniaxial stress
Journal of Biomechanical Science and Engineering
January 1, 2020
Heonuk JEONG, Xiaoguang YANG, Zihan PEI, Takashi USHIDA, Katsuko S. FURUKAWA
60 min, secondary antibody (Alexa Fluor 488,
A11029
,
Invitrogen
) solution with rhodamine phalloidin (
PHDR1
,
Cytoskeleton
) for 60 min, and DAPI (40,6-diamidino-2-phenylindole, Dojindo) for 5 min at room temperature
(71)
Small G protein signalling modulator 2 (SGSM2) is involved in oestrogen receptor-positive breast cancer metastasis through enhancement of migratory cell adhesion via interaction with E-cadherin
Cell adhesion & migration
December 1, 2019
Lin JH, Lee WJ, Wu HC, Wu CH, Chen LC, Huang CC, Chang HL, Cheng TC, Chang HW, Ho CT,
et al
.
from Jackson ImmunoResearch Lab Co. Phalloidin-iFluor 488 (
ab176753
,
Abcam
) or rhodamine phalloidin (#
PHDR1
,
Cytoskeleton Inc
.) were used for
staining
F-actin, which was performed according to the manufacturer’s
(72)
Knockdown of cytokeratin 8 overcomes chemoresistance of chordoma cells by aggravating endoplasmic reticulum stress through PERK/eIF2α arm of unfolded protein response and blocking autophagy
Cell death & disease
November 25, 2019
Wang D, Zhang P, Xu X, Wang J, Wang D, Peng P, Zheng C, Meng QJ, Yang L, Luo Z.
4′,6-diamidino-2-phenylindole (C1006, Beyotime Biotechnology, China) for 10 min or rhodamine phalloidin (#
PHDR1
,
Cytoskeleton, Inc
, USA) for 30 min. Each step was followed by washing with PBS three times for 5 min
(73)
Crumbs3 is a critical factor that regulates invasion and metastasis of colon adenocarcinoma
via
the specific interaction with FGFR1
International Journal of Cancer
November 15, 2019
Hidekazu Iioka, Ken Saito, Masakiyo Sakaguchi, Taro Tachibana, Keiichi Homma, Eisaku Kondo
performed for 1 hr at room temperature. Counterstain with Rhodamine‐Phalloidin (
1:1,000
dilution, #
PHDR1
,
Cytoskeleton
, USA) and/or Hoechst 33342 was done simultaneously with the secondary antibody reaction.
(74)
Arp2/3-Branched Actin Maintains an Active Pool of GTP-RhoA and Controls RhoA Abundance
Cells
October 16, 2019
Huang Y, Yi X, Kang C, Wu C.
488 secondary antibody (
A-21202
,
Life Technologies
, Carlsbad, CA, USA) and
Acti-stain 488 phalloidin
(
PHDR1
-A,
Cytoskeleton
, Denver, CO, USA) or Rhodamine Phalloidin (
PHDR1
,
Cytoskeleton
, Denver, CO, USA) for
(75)
Microphysiological sensing platform for an in-situ detection of tissue-secreted cytokines
Biosensors and Bioelectronics: X
October 1, 2019
Alejandro Hernández-Albors, Albert G. Castaño, Xiomara Fernández-Garibay, María Alejandra Ortega, Jordina Balaguer, Javier Ramón-Azcón
from Canvax (TBS, BR0042, Biotech, Spain). Rhodamine-Phalloidin 480 was purchased from
Cytoskeleton
(
PHDR1
, USA). The Bradford solution (BIO-RAD
protein assay
cat No.
500-0006
) was purchased from
BIO-RAD laboratories
(76)
Effect of Mechanical Compression on Invasion Process of Malignant Melanoma Using In Vitro Three-Dimensional Cell Culture Device
Micromachines (Basel)
September 30, 2019
Takashi Morikura, Shogo Miyata
room temperature. To stain F-
actin filaments
, the cells were incubated with 0.7% rhodamine-phalloidin (
PHDR1
,
Cytoskeleton Inc
, Denver, CO, USA) for 30 min at 37 °C. The F-
actin filaments
were visualized by an
(77)
RUNX1 promotes tumour metastasis by activating the Wnt/β-catenin signalling pathway and EMT in colorectal cancer
Journal of experimental & clinical cancer research : CR
August 1, 2019
Li Q, Lai Q, He C, Fang Y, Yan Q, Zhang Y, Wang X, Gu C, Wang Y, Ye L,
et al
.
were incubated in blocking solution at 4 °C overnight. Rhodamine phalloidin (
1:1500
,
Cytoskeleton
,
PHDR1
), which was used for detecting F-actin, was incubated in blocking solution at room temperature in the
(78)
Muscle-on-a-chip with an on-site multiplexed biosensing system for
in situ
monitoring of secreted IL-6 and TNF-α
Lab on a Chip
June 28, 2019
María A. Ortega, Xiomara Fernández-Garibay, Albert G. Castaño, Francesco De Chiara, Alejandro Hernández-Albors, Jordina Balaguer-Trias, Javier Ramón-Azcón
for 2 h. Afterwards, tissues were washed with TBS and incubated in Rhodamine-Phalloidin 480 (100 nM) (
PHDR1
,
Cytoskeleton Inc
, Colorado, USA) solution overnight to stain filamentous actin (F-actin). An additional
(79)
Effect of Cyclic Stretch on Tissue Maturation in Myoblast-Laden Hydrogel Fibers
Micromachines (Basel)
June 15, 2019
Shinako Bansai, Takashi Morikura, Hiroaki Onoe, Shogo Miyata
incubated with rhodamine-phalloidin buffered saline (PBS) for 5 min room temperature. cell fixation, the0.7% cell fibers were incubated ◦ (
PHDR1
,
Cytoskeleton
) for 30 min at 37 C. After fluorescent
staining
, the cells were by with 0.7% rhodamine-phalloidin
(80)
Resveratrol alleviates hypoxia/reoxygenation injury‑induced mitochondrial oxidative stress in cardiomyocytes
Molecular medicine reports
April 1, 2019
Li T, Chen L, Yu Y, Yang B, Li P, Tan XQ.
addition, for the
staining
of F‑actin, the NRCMs were treated with 100 nM rhodamine phalloidin (cat. no.
PHDR1
;
Cytoskeleton, Inc
., Denver, CO, USA) for 30 min at room temperature. The nuclei were stained with DAPI.
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Reviews
Write a Review
(1)
2/11/2019
Rhodamine Phalloidin for Actin Staining
Dr. Mohammad Alam
Our aim was to examine F-actin staining in cardiomyocytes. We transfected adult cardiomyocytes with the scramble and target specific siRNA. It was analyzed with F-actin specific antibody. Antibody is...
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Rhodamine Phalloidin (14uM in methanol) from Cytoskeleton, Inc.
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View Company Product Page
Pricing Info
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